Artificial insemination of guinea fowl

1. The main equipments include cleaning, disinfection, and drying equipment for the collection of fine cups, test tubes, microscopes, slides, coverslips, infusion droppers, diluents, alcohol, and instruments.

2. The spermatozoa adopts massage method and needs 2 to 3 people to complete it. One person can sit on a bench and put the head of the rooster head backwards, the chest is pressed on the leg and the abdomen and cloacal cavity are hung on the outside of the leg. The chest of the chicken can also be used. Place it directly on the bench, with legs hanging down on the stool; sometimes the chicken body can be clamped between the left elbow and the left waist. But in general, the force should be moderate. Another person rhythmically massaged several times with his right hand along the back and back of the cock, then the left thumb and the other four fingers quickly massaged and shaken on the two sides of the cloaca (around the anus), causing it to irritate and trigger the erection of the copulator. When the sperm is exhausted, the right hand quickly collects the semen with a sperm collecting device, and at the same time uses his left hand to squeeze on the two sides of the cloaca and promotes the ejaculation.

3, insemination In order to ensure the quality of semen, can be used to suck a drop of semen on the slide, add 1 drop of saline, cover the back after the cover glass, under low magnification microscope observation, identification of sperm vitality, density And quality. The semen meeting the requirements was pipetted into a test tube filled with a 0.9% physiological saline diluent, diluted 1:1, and the hens were inseminated in time. The insemination was completed by two people. One person grasped the hen's legs and lifted the abdomen inwards with his left hand. He pressed the tail of the hen with his right hand and opened the anus with separate thumb and forefinger to expose the fallopian tube. The mouth is on the upper left side of the cloaca, and the right side is the rectum opening). When the fallopian tube is completely opened, another person inserts the vas deferens obliquely into the fallopian tube by 2 to 3 centimeters, and slowly enters the semen.

4. Notes: The rooster usually collects sperm 2 times a week or 5 days in 1 day. The hens inseminate once every 5 days. The insemination volume of each hen was 0.013 to 0.015 ml of pure semen. Hens should be inseminated a few hours after laying or inseminated hours before the next day.

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