Quickly understand whether the fermenter is contaminated or not
1. Problems occur in the seed culture stage
2. Preparation and sterilization of the medium
3. There is a problem with the air filter
4. Operational problems during the cultivation process
Judging the fermenting tank
Abnormal change in state parameters
1. Abnormal changes in pH after infection
2. Abnormal changes in PO2 values
3. Abnormal changes in foam
4. Abnormal changes in OD values
Approximate judgment of the fermenting path of fermenter
1. Bacillus----sterilization is not complete
2. E. coli----contamination caused by operation
3. Cocci, Brevibacterium----Air
4. Phage - environmental reasons
Fermentation tank conventional treatment
1. Thoroughly clean the tank
2. Replace the air intake filter and exhaust filter
3. Check the status during sterilization
4. Perform 24-hour aseptic culture to eliminate the cause of tank sterilization
5. Environmental disinfection
6. Strict operation procedures
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