CRISPR PK RNAi Who are you more optimistic about?
As a gene in the field of gene silencing, RNAi has also been used in the past few years. It has been named one of the top ten breakthrough technologies of the year by Science. However, due to the emergence of the rising star CRISPR in recent years, the RNAi scenery no longer seems to have fallen from the peak of the expected peak to the bottom of the disillusionment. Is RNAi really OUT? How do we choose between RNAi and CRISPR?
RNAi generally introduces siRNA or ShRNA into a cell (which can form siRNA after being cleaved by Dicer enzyme), thereby degrading the corresponding mRNA, and performing gene screening by a functional deletion phenotype.
It can be seen that RNAi mainly targets mature RNA, which is a gene knockdown method, but this technique cannot completely remove the function of the gene.
The true loss of complete function of the gene is the zinc finger enzymes ZFN and TALEN, which recognize specific DNA sequences through the DNA binding domain (DBD) and introduce double-strand breaks (DSBs) and mutations into the target gene using nucleases. Knock out the role of genes. However, the brilliance of ZFN and TALEN was quickly overshadowed by the sudden emergence of the CRISPR system.
CRISPR/Cas9 acts as a bacterial immune system that targets and cleaves with a nucleic acid fragment of interest (whether or not it has a coding function) under the guidance of a guide RNA.
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