Ten years of embryos successfully thawed and conceived
Release date: 2015-12-28
Ten years of frozen embryo transfer success
Last week, Wang Fang (pseudonym) went to the hospital to take blood and test pregnancy. The result came out very quickly. The blood HCG was positive and she was pregnant. Wang Fang’s heart that has been hanging for half a month has finally fallen. For a 40-year-old woman, it is a magical and embarrassing experience to harbor her own frozen embryo ten years ago.
Ten years ago, Wang Fang and her husband went to the reproductive center of the 181st Hospital of the People's Liberation Army for infertility. After diagnosis, her fallopian tube was blocked and her husband's semen was abnormal. In this case, it is impossible to naturally conceive and have children. A test tube baby was made at the hospital reproductive center.
After taking a few procedures, such as taking eggs, taking sperm, in vitro fertilization, embryo transfer, etc., the hospital helped Wang Fang couple five high-quality embryos. At that time, two transplanted into Wangfang uterus, the remaining three embryos were frozen. Deposited into the liquid nitrogen tank of the hospital's reproductive center.
After the transplant, the embryos were successfully implanted. Ten months later, Wang Fang gave birth to a healthy big fat boy. Like many parents, Wang Fang and his wife wanted to regenerate a good companion after they had given birth to their first child. However, because the policy was not allowed at the time, they had to break this thought.
In 2014, the state approved a separate two-child, Wang Fang and his wife compared the following conditions, and moved the idea of ​​regenerating one. But at this time, Wang Fang had nearly forty years old and had already passed the golden age of birth. So she remembered the remaining three embryos that were frozen in the hospital that year. She called the hospital's reproductive center and was very fortunate. When the couple came to the hospital to confirm the abandonment of the frozen embryo, although the hospital had not paid the deposit fee for ten years, the reproductive center was still responsible for the patient. The embryos were preserved, and the three embryos ten years ago were well preserved in a liquid nitrogen tank at -196 °C.
When Wang Fang wanted to come, although the frozen embryos were well preserved ten years ago, it was incredible to use the embryos ten years ago to harbor the baby, and she was entangled. The doctor gave a suggestion that with the increase of age, the ovarian function is significantly reduced, the egg quality is declining, and the risk of chromosomal abnormalities in the embryo is increased. I want her to use the embryo obtained when she is nearly 10 years old. From the perspective of prenatal and postnatal education, it may be better than the quality of the embryos she is currently seeking to obtain eggs.
After repeated thoughts, Wang Fang and his wife decided to give a try. After the birth certificate was completed, Wang Fang and his wife went to the reproductive center to request embryo thawing transplant in October this year. According to Wang Fang's situation, the center developed a personalized embryo resuscitation transplant program for her.
Half a month ago, Wang Fang's physical conditions and other aspects were ready. The center took out her frozen embryos from the laboratory's liquid nitrogen tank, and used embryo thawing technology to successfully recover the embryos that had been sleeping for 10 years. Two embryos entered the palace of Wangfang. Half a month later, 40-year-old Wang Fang succeeded in getting pregnant again.
Magical frozen embryo technology
In ordinary people's homes, there are refrigerators frozen chickens and frozen fish, but people know that frozen things must be dead, even if they are frozen, they can't survive. Because of this conventional experience, frozen embryos are thawed and awakened. It makes people feel amazing. How is the embryo frozen and thawed, and how is the process different from our usual refrigerator cold solution? The reporter walked into the laboratory of the Reproductive Center of the 181st Hospital of the People's Liberation Army and learned the magic of this technology.
The freezer in the lab is not a big hail as one might imagine, but a room with lots of liquid nitrogen tanks. These liquid nitrogen tanks are the size of the water tank. Liu Ying, the deputy director of the reproductive center and the head of the laboratory, said that these liquid nitrogen tanks are made of special materials and are completely enclosed. The temperature inside the tank is kept at -196 ° C for many years, and there are 30,000 frozen in the tank. The remaining embryos.
"We know that cells metabolize at room temperature to produce energy, discharge waste, and grow continuously. But when the cells are placed in an ultra-low temperature environment of -196 ° C, the cells stop metabolism, stop growing, and save, regardless of ten years or centuries. There will be no change.†Liu Ying, deputy director, said that after the use of ovulation-promoting drugs, there will often be many high-quality embryos. In addition to 2-3 transplants, the remaining high-quality embryos can be cryopreserved, and then thawed and transplanted later. The opportunity to conceive, or some patients with severe ovarian hyperstimulation syndrome and other special causes that cannot be transplanted during the treatment cycle, can also freeze the embryos for later resuscitation transplantation.
Embryo freezing and resuscitation transplantation techniques are divided into two types, one is the traditional slow-program freezing, and the other is the more advanced rapid vitrification technology. The vitrification technology can avoid the physical and chemical damage of ice crystals to cells, and can obtain better freezing effect, and the glass freezing and thawing technology takes a short time and the embryo recovery rate is high. Before 2009, the reproductive center used the program freezing technology. According to the statistics of the hospital, the success rate of transplantation after resuscitation of frozen embryos with old technology is only 20%, and the success rate of resuscitation of embryos with new technology is over 98%. The pregnancy rate can be as high as 70%. Wang Fang used the old technology frozen embryo ten years ago. This time, the successful thawing transplant pregnancy is the lucky one in the 20% success rate.
Of course, there are many couples who worry that the embryo will be frozen for a long time, will the quality of the embryo be bad, and will the baby born be unhealthy? Reproductive center experts suggest that, in fact, when the embryo is frozen, the embryonic experimenter selects the embryo, and only the embryos of good quality that meet the cryopreservation standard can be cryopreserved. Once stored in liquid nitrogen at -196 ° C, the embryo is in a "sleeping" state, as long as it is not resuscitated, it will remain in the state of cryopreservation. When the embryo is thawed and revived, a few less powerful embryos are in During the recovery process, it will be eliminated, and the embryos that have been successfully resuscitated are the excellent quality embryos that have been carefully screened, have the potential for bed and smooth and healthy development.
Focus on reproductive technology for more than 10,000 people
The frozen embryo resuscitation technique is amazing, but it was discovered after entering the Reproductive Center of the 181st Hospital of the People's Liberation Army that this is only one of the many advanced reproductive technologies in the center. The center was established on November 6, 2005. For ten years, the center has focused on reproductive technology. I have completed many "impossible" that I could not have thought of ten years ago. This also brought a lot of surprises and help to the people.
In December 2005, the routine IVF of the Reproductive Center was successfully assisted; in 2006, the spermatozoa of the reproductive center was successfully injected into the intracytoplasmic injection; in December 2009, it became the first army to pass the review to grant assisted reproductive technology. The Reproductive Center is also the only reproductive center in northern Guangxi that can simultaneously carry out various assisted reproductive technologies such as artificial insemination, artificial insemination, in vitro fertilization-embryo transfer and single sperm intracytoplasmic injection. Since its establishment in 2006, the center has received nearly one million infertility patients at home and abroad. Up to now, it has carried out more than 20,000 cycles of assisted reproductive technology, and has more than 10,000 healthy babies. It is the assisted reproductive technology of the whole region and the whole army. One of the reproductive centers with the most cycles.
With the continuous expansion of business volume, the Reproductive Center added a second laboratory in early 2014 and successfully put into operation, increasing the pressure on the center to increase the number of assisted pregnancy cycles, and also solving the need for laboratories and equipment in previous years. The annual examination has to stop the surgery. In 2014, the IVF assisted pregnancy cycle reached 4000. The success rate of IVF in the reproductive center was stable at more than 60%, and the success rate of thawing transplantation for women under 35 years old was about 70%.
At the end of 2014, the Center launched “pre-plant genetic screening†(third-generation IVF technology), which is another important milestone in reproductive technology upgrades for the entire center. According to Duan Jinliang, director of the center, the center often encounters many special patients, such as elderly patients, chromosomal abnormalities, and patients with recurrent miscarriage. They are less likely to be pregnant through natural conception or common "test tube baby". And after pregnancy, the fetus is prone to miscarriage, deformity, etc. during the growth and development process, and it is difficult to obtain a healthy baby. In these cases, the first-generation and second-generation IVF technologies cannot be solved, or some patients with unexplained repeated IVF failures. To help them give birth, they have to upgrade their skills.
The "third generation IVF" technology is based on in vitro fertilization embryo transfer technology, genetic diagnosis (PGD) and genetic screening (PGS) of the embryo before implantation, ie the number of chromosomes or results of the embryo Screening, analysis of whether there is abnormality of genetic material, inferior storage, selection of normal embryos for transplantation, thereby achieving the purpose of increasing pregnancy rate, reducing abortion rate, and reducing birth defects. According to reports, this technology was successful in July 2015. The first patient was a 37-year-old woman with a history of 3 spontaneous abortions. She was screened for "quality" embryos by PGS technology and successfully conceived. Prenatal diagnosis of the fetus was normal. It is now a risk period for early abortion and has been pregnant for 5 months.
On the occasion of the 10th anniversary of the birth center, Duan Jinliang was quite touched as the helm of the entire team. “The center was established 10 years ago. At that time, people did not know much about reproductive technology. The number of outpatients in the first year was less than 6,000. With the continuous innovation of our technology, the dream of many people has been fulfilled, and the center has been recognized by many people. Since the development, the annual outpatient volume has increased to 150,000. In the future, the center will continue to work hard to continuously improve itself with technological innovation. Strength, serving the people."
Source: Guilin Evening News
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