The combination of CRISPR gene editing and RNA-seq can advance medical advancement
What combination of mutations can help cancer cells survive? Which cells in the brain are involved in the onset of Alzheimer's disease? How do immune cells make intricate decision-making processes? Now, researchers at the Weizmann Institute of Science in Israel combine two powerful research tools—CRISPR gene editing and single-cell genomic analysis—in one method to help us answer these questions and other unanswered questions.
What combination of mutations can help cancer cells survive? Which cells in the brain are involved in the onset of Alzheimer's disease? How do immune cells make intricate decision-making processes? Now, researchers at the Weizmann Institute of Science in Israel combine two powerful research tools—CRISPR gene editing and single-cell genomic analysis—in one method to help us answer these questions and other unanswered questions.
This new technology allows researchers to manipulate gene function in a single cell and understand the results of each change with extremely high resolution. Scientists say that a single experiment using this method may be comparable to thousands of experiments performed using previous methods, which may drive the advancement of genetic engineering in the medical field.
CRISPR gene editing technology has changed the world of biological research, and its clinical application in humans is just around the corner. For the first time, CRISPR is found in bacteria. As an original acquired immune system, it can cut and paste viral DNA into its own genome to fight the virus. In recent years, researchers have used the bacterial system to quickly and efficiently clip or insert almost any gene into any organism or cell. The author of the study, Professor Ido Amit of the Department of Immunology at the Weizmann Institute of Science, said: "But for the CRISPR itself, it is a blunt research tool because we often have difficulty observing or understanding the editing of this genome. "The results." Dr. Diego Jaitin of Amit Labs added: "So far, most studies have been looking for non-black and white effects, but most of the processes in the body are complex and even confusing."
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