Problems and Countermeasures in the Cultivation of Unicellular Algae

Due to the severe depletion of offshore fishery resources, the production of natural fish and shrimp can no longer meet the market's demand. Emerging seafood breeding and factory farming are increasingly becoming the leading industries, while the basic culture of bait is the cultivation of seedlings. In order to ensure the smooth progress of the cultivation of single-cell algae, the important issues and countermeasures in the algae cultivation process are described below:

First, must buy high-quality algae species

At the time of purchase, through the appearance and microscopic examination of the algae fluid, the algae fluid that has grown vigorously without microorganisms, predator organisms and algae is contaminated.

Second, try to avoid algae pollution in culture

1. Breeding room

The main source of pollution is bacteria, so we should pay attention to disinfection to ensure good growth of algae in the bottle. Adhere to the daily ground disinfection; each time before shaking the bottle, inoculate with soap hand wash, and then use alcohol to disinfect, in order to avoid bacterial contamination of the algae species; inoculation with conical flasks, bottles must be thoroughly disinfected; cap bottle The white paper on the mouth must also be kept clean and replaced on a regular basis; non-workers must not enter the seed-keeping room to avoid introducing bacteria.

If the bottle has been contaminated with bacteria, the large number of bacteria will cause the algal cells to grow slowly and sink a lot. Add 10,000 international units of penicillin to 1 liter of algae to effectively inhibit the growth of bacteria.

2. In the workshop

The main sources of pollution are bacteria, rotifers, and small protozoa, so we should pay attention to: (1) The brush pool must be completely and completely remove the pollution sources stored in the pool; (2) Operation must be standardized to avoid cross-infection between pools and artificially brought into the source of pollution . If the enemy organisms in the pond have affected the growth of algae, measures can be taken as follows: (1) Use filtering methods to remove large enemy organisms (such as rotifers, etc.), and use a 60-μm mesh sieve to filter continuously for 3 consecutive days. Once; (2) 10 ppm to 15 ppm of available Chlorococcus algae and small white insects; (3) 0.03% of medical conc. ammonia, which can effectively kill the flatworms of Planophyta and Spirulina; (4) Salts that can be used when amoeba are harmful The characteristics of halophilic algae, in the algae by adding appropriate amount of salt, can kill amoeba or other small protozoa, the contaminated culture medium will change from yellow-green to bright green, strong growth; (5) Platycladi, gold algae Occurrence of tip insects and Euphorbia species can be achieved by acidification with hydrochloric acid. Add 1 mol of hydrochloric acid (ie, pure hydrochloric acid mixed with water in a ratio of 1:11) to the algae, and stir while stirring. When the algae pH value It was reduced to 3, hydrochloric acid was added, acidified for 1 hour, and then neutralized with 1 mol of sodium hydroxide to the original pH range. On the next day, the algae fluid was able to resume swimming. After a period of cultivation, the algae grew normally.

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