Sterilization, inoculation and culture of mushroom medium
I. Sterilization of the sterilizing medium of the culture medium is the key to the success of the strain production. There are a large number of microorganisms in the non-sterilized medium, which often leads to contamination of strains and causes losses to the production. Currently, the mushroom farmer The commonly used sterilization method is atmospheric steam sterilization. Atmospheric pressure steam sterilized atmospheric pressure sterilized stoves are made of bricks, selected 110 cm diameter wok, with a brick height 100 cm, a diameter of 120 cm drum, smooth with cement wipes inside and outside. After the medium is filled in the sterilizing pot, cover the mouth of the pot with a thicker plastic film and sack cloth, sturdy with a rope, and then burn it vigorously to boil the pot in the shortest time. After the plastic film is blown into a steamed bun, it indicates that the pan has been boiled. The sterilization time starts from this time and lasts for 8 to 10 hours to ensure thorough sterilization. Some mushroom farmers are often paralyzed on sterilization. Bacterial contamination has led to failure of seed production. In the process of atmospheric pressure sterilization, “three preventions†must be implemented. First, it is necessary to prevent cooling in the middle of the process. During the middle period, it is necessary to stop the fire. If the pot reaches less than 100° C., sterilization is not achieved within the specified time. Second, to prevent burning pan, in the pot before sterilization to add enough water, in the sterilization process, if the pot is not enough water, water injection from the water inlet. Third, we must prevent the presence of sterile corners, such as the uneven spot at the end of the fire, the mouth of the cylinder leak only from one end, etc., sterilization blind corner may occur. Second, inoculation and culture After the sterilization of the medium can be inoculated, inoculation is to transplant the bacteria on the culture medium. Inoculation operators must establish a strict sterility concept, leaving operators and vaccination tools in a strictly sterile state. Therefore, the inoculation tool must be strictly sterilized, and the operating environment must be thoroughly disinfected. The operator's hands and clothing must also be disinfected and strict compliance with the aseptic procedures. The inoculation is carried out in the inoculation box or in the inoculation room. When inoculating, first clear the inoculation box or inoculation room debris, spray disinfection with 2% of the Soviet Union children. The medium to be inoculated, strains, and tools used are placed on the inoculation box or on the inoculation room table, and the inoculation box and the inoculation room are fumigated with formalin and potassium permanganate for at least half an hour. The amount is per cubic meter of space. With 5 grams of potassium permanganate, add 10 ml of formaldehyde. Before inoculation, use 75% alcohol to wipe hands, mother tubes, or the appearance of the original bottle, and then light the alcohol lamp. After the inoculation is completed, the alcohol lamp is extinguished and the inoculated bottle or bag is moved to the culture room for cultivation. In general, one parent species can be used to expand the original species of 4-5 bottles, and one bottle of original species can be used to expand the cultivation of 40-50 bottles or 15-20 bags. The culturing room should be kept clean and adjusted to temperature, humidity, air, light and other environmental conditions. The suitable temperature for strain culture should be 20-25°C, and the relative humidity of air should be controlled at about 60%. During the germination period, it requires dark-light germs, and the culture room should always maintain a well-ventilated environment. According to this management method, strains can be filled with bottles or bags in about 40 days. If strains that are full of bottles (bags) are not used immediately, they should be stored in an environment below 0-10°C and can be preserved for 1-3 months. (Source: Zhejiang Rural Network)
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